your girlfriend and/or your wife: act one
act two: racist jokes (if youre a minority, dispense with the stereotypes)
act three: drugs. if you are clean, fantasy drugs. alcohol sort of counts, but you have to talk about consuming it intravenously. or enemas. beer enemas
act four: sex.. or lack thereof. penis sizes come to mind, but if you already delved a lot in act one or two, ski this part.
depending on the state of your act at this time, improv with the audience.. or stare at them for an uncomfortably long time
act six: politics/pop-culture humor. again, depends on your schtick. you can go for current pop culture or nostalgia. don't go too far though. not a lot of people still get Laverne and Shirley.
act seven: dispense with memes.. if you got the balls; which is assumed you have since you managed to get to this stage without getting thrown out after act two.